Chaihu, a traditional Chinese medicine name. It is a medicinal herb included in the Chinese Pharmacopoeia. The medicinal part is the dried root of Bupleurum chinense, a plant of Apiaceae, or the narrow leaf Bupleurum chinense. Harvest in spring and autumn, remove stems, leaves, and sediment, and dry. Chaihu is a commonly used surface relieving medicine. Also known as Dixun, Shancai, Mushroom Grass, and Chaicao, it has a bitter and slightly cold taste, and belongs to the liver and gallbladder meridians. It has the effect of reconciling the exterior and interior, soothing the liver and elevating yang. For cold and fever, cold and heat exchange, malaria, liver depression and qi stagnation, chest and rib pain, prolapse of anus, Uterine prolapse, irregular menstruation.
Take 1g of this product and dissolve it in 30ml of water. Shake and extract twice with ether, 15ml each time. Discard the ether solution. Shake the water solution with water saturated n-butanol twice, 20ml each time. Combine the n-butanol solution, add 40ml of ammonia test solution, shake well, place to layer, separate the n-butanol solution, evaporate to dryness, add 1ml of methanol to dissolve the residue, and use it as the test solution. Take another 0.5g of Chaihu reference medicinal material, add 20ml of methanol, heat and reflux at 80 ° C for 1 hour, cool, filter, evaporate the filtrate, and dissolve the residue in 10ml of water. Prepare a reference medicinal material solution using the same method. Carry out the Thin-layer chromatography test, draw the test solution, 8ul, and the control medicine solution, 4ul, respectively, and dab them on the same silica gel G thin layer plate, develop them with the lower layer solution developer placed below 10 ℃ in chloroform methanol water (13:7:2), take them out, dry them in the air, spray 1% paracetamol Benzaldehyde sulfuric acid ethanol solution (1 → 10), and heat them until the spots are clear. In the chromatogram of the test sample, the main spots of the same color appear at the corresponding positions of the reference medicinal material chromatogram; When viewed under ultraviolet light (365nm), the same yellow fluorescent spots appear.