Goutenine, brown powder. Extracted from Uncaria macrophylla Wall., a Rubiaceae plant. Melting point 216 ℃. Optical rotation -14.7 ° (c=2.5, chloroform). Soluble in chloroform, soluble in acetone, ethanol, benzene, slightly soluble in ether and ethyl acetate, insoluble in Petroleum ether. Mobile phase: methanol water (55:45), containing 0.01 mol/L triethylamine, adjusted to pH 7.5 with glacial acetic acid. Flow rate: 1.0 mL/min, detection wavelength 254nm.
Extraction and Storage of Goutenine
Extraction source of rhynchophylline: Uncaria macrophylla Wall., Rubiaceae.
Extraction method of Gouteng alkaloid:
1. The alkaline benzene extraction method can extract rhynchophylline, and after treatment, high-purity rhynchophylline can be obtained. This process is simple and feasible, suitable for large-scale production.
2. Extract using alkaloid reflux extraction method.
3. Preparation of crude extracts from Uncaria lobata by low grade alcohol water cold immersion ultrasonic method for pre-treatment; Using two organic reagents and a certain proportion of ammonia water as the eluent, the crude extract was separated by silica gel column chromatography; The eluted fraction was extracted and purified to obtain the monomers of rhynchophylline. This method is safe, efficient, low cost, and has a high yield of rhynchophylline monomers. 0.27g of rhynchophylline monomers can be obtained per kilogram of medicinal herbs, with a purity higher than 90%, which is conducive to industrial large-scale production and provides a way for further development of rhynchophylline.
Packaging method: 25kg/cardboard bucket or aluminum foil bag, packaged as needed
Storage conditions: It should be sealed and shaded, and stored in a dry, cool, and well ventilated area. Avoid incompatible substances, flammable materials, etc.